aria2 bcftools bedtools bioawk blast bowtie2 bwa csvkit csvtk datamash emboss fastp fastqc freebayes hisat2 jq mafft make>4 minimap2 ncbi-datasets-cli parallel perl-text-csv samtools>=1.14 seqkit seqtk snpeff==5.0 subread trimmomatic ucsc-bedgraphtobigwig wget