Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	475,288:1	+G	100%	coding (18/1677 nt)	ybaL ←	Kef family K(+) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	475,288	1	.	G	100.0%	11.6 / NA	5	P6P (CCG→CCC)	ybaL	Kef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (1/4);  total (1/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

GCACAAGGCCGCCAACAATGGTGGTGATAAGC‑GGGGTGGCGTGATGCATTCCGTCTCCTTTT  >  NC_012967/475257‑475318                                 |                               gCACAAGGCCGCCAACAATGGTGGTGATAAGC‑gggg                            <  2:761601/36‑1 (MQ=255)            ccAACAATGGTGGTGATAAGCGGGGGTGGCGTGATg                  <  2:415898/36‑1 (MQ=37)                    ggtggtGATAAGCGGGGGTGGCGTGATGCATTCCGt          <  2:126244/36‑1 (MQ=37)                    ggtggtGATAAGCGGGGGTGGCGTGATGCATTCCGt          <  2:700197/36‑1 (MQ=37)                           aTAAGCGGGGGTGGCGTGATGCATTCCGTCTCCttt   >  1:359037/1‑36 (MQ=37)                            tAAGCGGGGGTGGCGTGATGCATTCCGTCTCCtttt  <  2:547277/36‑1 (MQ=37)                                 |                               GCACAAGGCCGCCAACAATGGTGGTGATAAGC‑GGGGTGGCGTGATGCATTCCGTCTCCTTTT  >  NC_012967/475257‑475318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑