BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 161,041 T→G 100% N313H (AAC→CAC)  pcnB ← polynucleotide adenylyltransferase PcnB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012967161,0410TG100.0% 30.6 / NA 12N313H (AAC→CAC) pcnBpolynucleotide adenylyltransferase PcnB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/6);  total (6/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT  >  NC_012967/161011‑161068
                              |                           
aGAACATGGCGGCAAACAGGAACGCCGGGTGCAcgc                        >  1:707186/1‑36 (MQ=38)
 gAACATCGCGGCAAACAGGAACGCCGGCTGCAcgcg                       >  2:553187/1‑36 (MQ=16)
       ggcggcAAACAGGAACGCCGGGTGGACGCGCATATc                 >  2:598552/1‑36 (MQ=25)
        gcggcAAACAGGAACGCCGGGTGCACGCGCATATCg                >  2:342227/1‑36 (MQ=38)
         cggcAAACAGGAACGCCGGGTGCACGCGCACATCGt               >  2:112526/1‑36 (MQ=25)
          ggcAAACAGGAACGCCGGGTGCACGCGCATATCGtt              <  2:857942/36‑1 (MQ=38)
            cAAACAGGAACGCCGGGTGCACGCGCATATCGTTAt            <  2:73563/36‑1 (MQ=39)
              aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg          <  1:414799/36‑1 (MQ=39)
              aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg          <  2:234382/36‑1 (MQ=39)
                     aggCCGGGTGCACGCGCATATCGTTATGGATAcgcg   <  1:224693/34‑1 (MQ=37)
                     aCGCCGGGTGCACGCGCATATCGTTATGGATAggcg   >  2:590726/1‑36 (MQ=37)
                      cGCCGGGTGCACGCGCATATCGTTATGGATACGCGt  <  2:953052/36‑1 (MQ=37)
                              |                           
AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT  >  NC_012967/161011‑161068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: