Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	161,041	T→G	100%	N313H (AAC→CAC)	pcnB ←	polynucleotide adenylyltransferase PcnB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	161,041	0	T	G	100.0%	30.6 / NA	12	N313H (AAC→CAC)	pcnB	polynucleotide adenylyltransferase PcnB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/6);  total (6/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT  >  NC_012967/161011‑161068                               |                            aGAACATGGCGGCAAACAGGAACGCCGGGTGCAcgc                        >  1:707186/1‑36 (MQ=38)  gAACATCGCGGCAAACAGGAACGCCGGCTGCAcgcg                       >  2:553187/1‑36 (MQ=16)        ggcggcAAACAGGAACGCCGGGTGGACGCGCATATc                 >  2:598552/1‑36 (MQ=25)         gcggcAAACAGGAACGCCGGGTGCACGCGCATATCg                >  2:342227/1‑36 (MQ=38)          cggcAAACAGGAACGCCGGGTGCACGCGCACATCGt               >  2:112526/1‑36 (MQ=25)           ggcAAACAGGAACGCCGGGTGCACGCGCATATCGtt              <  2:857942/36‑1 (MQ=38)             cAAACAGGAACGCCGGGTGCACGCGCATATCGTTAt            <  2:73563/36‑1 (MQ=39)               aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg          <  1:414799/36‑1 (MQ=39)               aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg          <  2:234382/36‑1 (MQ=39)                      aggCCGGGTGCACGCGCATATCGTTATGGATAcgcg   <  1:224693/34‑1 (MQ=37)                      aCGCCGGGTGCACGCGCATATCGTTATGGATAggcg   >  2:590726/1‑36 (MQ=37)                       cGCCGGGTGCACGCGCATATCGTTATGGATACGCGt  <  2:953052/36‑1 (MQ=37)                               |                            AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT  >  NC_012967/161011‑161068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑