Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 161,041 | T→G | 100% | N313H (AAC→CAC) | pcnB ← | polynucleotide adenylyltransferase PcnB |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 161,041 | 0 | T | G | 100.0% | 30.6 / NA | 12 | N313H (AAC→CAC) | pcnB | polynucleotide adenylyltransferase PcnB |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (6/6); total (6/6) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT > NC_012967/161011‑161068 | aGAACATGGCGGCAAACAGGAACGCCGGGTGCAcgc > 1:707186/1‑36 (MQ=38) gAACATCGCGGCAAACAGGAACGCCGGCTGCAcgcg > 2:553187/1‑36 (MQ=16) ggcggcAAACAGGAACGCCGGGTGGACGCGCATATc > 2:598552/1‑36 (MQ=25) gcggcAAACAGGAACGCCGGGTGCACGCGCATATCg > 2:342227/1‑36 (MQ=38) cggcAAACAGGAACGCCGGGTGCACGCGCACATCGt > 2:112526/1‑36 (MQ=25) ggcAAACAGGAACGCCGGGTGCACGCGCATATCGtt < 2:857942/36‑1 (MQ=38) cAAACAGGAACGCCGGGTGCACGCGCATATCGTTAt < 2:73563/36‑1 (MQ=39) aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg < 1:414799/36‑1 (MQ=39) aaCAGGAACGCCGGGTGCACGCGCATATCGTTATgg < 2:234382/36‑1 (MQ=39) aggCCGGGTGCACGCGCATATCGTTATGGATAcgcg < 1:224693/34‑1 (MQ=37) aCGCCGGGTGCACGCGCATATCGTTATGGATAggcg > 2:590726/1‑36 (MQ=37) cGCCGGGTGCACGCGCATATCGTTATGGATACGCGt < 2:953052/36‑1 (MQ=37) | AGAACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGT > NC_012967/161011‑161068 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |