BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 1,286,699 C→A 100% intergenic (+23/+161) narI → / ← ECB_RS22925 respiratory nitrate reductase subunit gamma/RtT sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129671,286,6990CA100.0% 28.7 / NA 11intergenic (+23/+161)narI/ECB_RS22925respiratory nitrate reductase subunit gamma/RtT sRNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/8);  total (3/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA  >  NC_012967/1286672‑1286727
                           |                            
aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg                      <  1:100917/36‑1 (MQ=39)
aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg                      >  2:574102/1‑36 (MQ=39)
   aaGCGAATTTTAGTTCACATAGACACTGCTTcggcg                   <  2:762477/36‑1 (MQ=39)
       gtaTTTTAGTTCACATAGACACTGCTTCGGCGGGGt               <  1:454/34‑1 (MQ=38)
       gAATTTTAGTTCACATAGACACTGCTTCGGCGGGGt               <  2:796928/36‑1 (MQ=39)
        aaTTTTAGTTCGCATAGACACTGCTTCGGCGGGGtt              <  2:426801/36‑1 (MQ=25)
         aTTTTAGTTCACATAGACACTGCTTCGGCGGGGttt             >  1:690117/1‑36 (MQ=38)
          ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt            <  1:187094/36‑1 (MQ=37)
          ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt            <  1:974897/36‑1 (MQ=37)
                 tCACATAGACACTGCTTCGGCGGGGTTTTTTTATgg     <  1:647771/36‑1 (MQ=37)
                    caTAGACACTGCTTCGGCGGGGTTTTTTTATGGGCa  >  2:680/1‑36 (MQ=37)
                           |                            
ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA  >  NC_012967/1286672‑1286727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: