Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	1,286,699	C→A	100%	intergenic (+23/+161)	narI → / ← ECB_RS22925	respiratory nitrate reductase subunit gamma/RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	1,286,699	0	C	A	100.0%	28.7 / NA	11	intergenic (+23/+161)	narI/ECB_RS22925	respiratory nitrate reductase subunit gamma/RtT sRNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/8);  total (3/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA > NC_012967/1286672‑1286727 | aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg < 1:100917/36‑1 (MQ=39) aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg > 2:574102/1‑36 (MQ=39) aaGCGAATTTTAGTTCACATAGACACTGCTTcggcg < 2:762477/36‑1 (MQ=39) gtaTTTTAGTTCACATAGACACTGCTTCGGCGGGGt < 1:454/34‑1 (MQ=38) gAATTTTAGTTCACATAGACACTGCTTCGGCGGGGt < 2:796928/36‑1 (MQ=39) aaTTTTAGTTCGCATAGACACTGCTTCGGCGGGGtt < 2:426801/36‑1 (MQ=25) aTTTTAGTTCACATAGACACTGCTTCGGCGGGGttt > 1:690117/1‑36 (MQ=38) ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt < 1:187094/36‑1 (MQ=37) ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt < 1:974897/36‑1 (MQ=37) tCACATAGACACTGCTTCGGCGGGGTTTTTTTATgg < 1:647771/36‑1 (MQ=37) caTAGACACTGCTTCGGCGGGGTTTTTTTATGGGCa > 2:680/1‑36 (MQ=37) | ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA > NC_012967/1286672‑1286727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑