Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 1,286,699 | C→A | 100% | intergenic (+23/+161) | narI → / ← ECB_RS22925 | respiratory nitrate reductase subunit gamma/RtT sRNA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 1,286,699 | 0 | C | A | 100.0% | 28.7 / NA | 11 | intergenic (+23/+161) | narI/ECB_RS22925 | respiratory nitrate reductase subunit gamma/RtT sRNA |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (3/8); total (3/8) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA > NC_012967/1286672‑1286727 | aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg < 1:100917/36‑1 (MQ=39) aCTAAGCGAATTTTAGTTCACATAGACACTGCTTcg > 2:574102/1‑36 (MQ=39) aaGCGAATTTTAGTTCACATAGACACTGCTTcggcg < 2:762477/36‑1 (MQ=39) gtaTTTTAGTTCACATAGACACTGCTTCGGCGGGGt < 1:454/34‑1 (MQ=38) gAATTTTAGTTCACATAGACACTGCTTCGGCGGGGt < 2:796928/36‑1 (MQ=39) aaTTTTAGTTCGCATAGACACTGCTTCGGCGGGGtt < 2:426801/36‑1 (MQ=25) aTTTTAGTTCACATAGACACTGCTTCGGCGGGGttt > 1:690117/1‑36 (MQ=38) ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt < 1:187094/36‑1 (MQ=37) ttttAGTTCACATAGACACTGCTTCGGCGGGGtttt < 1:974897/36‑1 (MQ=37) tCACATAGACACTGCTTCGGCGGGGTTTTTTTATgg < 1:647771/36‑1 (MQ=37) caTAGACACTGCTTCGGCGGGGTTTTTTTATGGGCa > 2:680/1‑36 (MQ=37) | ACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCA > NC_012967/1286672‑1286727 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |