| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_012967 | 1,329,516 | C→T | 100% | H33Y (CAC→TAC) | topA → | type I DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 1,329,516 | 0 | C | T | 100.0% | 49.9 / NA | 19 | H33Y (CAC→TAC) | topA | type I DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/11); total (8/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGC > NC_012967/1329484‑1329547 | gTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca < 2:536308/36‑1 (MQ=37) gTGACTACGTGGTGAAATCCAGCGTCGGGTACATcc > 2:638003/1‑36 (MQ=25) tGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg < 2:917386/36‑1 (MQ=37) tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAt > 1:961844/1‑36 (MQ=37) tACGTGGTGAAATCCAGCGTCGGGTACATTCGCGAt > 1:770289/1‑36 (MQ=16) cGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt < 2:741418/36‑1 (MQ=37) ggtgAAATCCAGCGTCGGTTTCATCCGCGGTTTGcc > 2:196985/1‑36 (MQ=16) ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGcc < 1:442916/36‑1 (MQ=38) gtgAAATCCAACGTCGGTTACATCCGCGATTTGCCg < 1:56434/36‑1 (MQ=37) aaaTCCAGCGTCGGTTACAACCGCGATTTGCCGAcc > 1:904040/1‑36 (MQ=25) aTCCAGCTTCGGTTACATCCGCGATTTGCCGACCAg < 2:859986/36‑1 (MQ=37) aTCCACCGTCGGTTACATCCGCGATTTGCCGACCAg < 2:523755/36‑1 (MQ=37) ccAGCGTCGGTTACATCCGCGATTTTTCGACCAGTg > 2:466689/1‑36 (MQ=19) ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTg < 1:589067/36‑1 (MQ=39) ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTg < 1:502295/36‑1 (MQ=39) gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt < 2:583720/36‑1 (MQ=39) cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc > 1:636767/1‑36 (MQ=39) cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc < 2:39012/36‑1 (MQ=39) tCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg < 1:576345/36‑1 (MQ=39) cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc > 2:886622/1‑36 (MQ=39) | GTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGC > NC_012967/1329484‑1329547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |