BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 1,976,879 T→G 100% intergenic (‑57/‑76) hprR ← / → hiuH response regulator transcription factor HprR/hydroxyisourate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129671,976,8790TG100.0% 32.6 / NA 11intergenic (‑57/‑76)hprR/hiuHresponse regulator transcription factor HprR/hydroxyisourate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/3);  total (8/3)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTATAGAAACAGCCTGGTTCATTACAAAATTGTAATGCTGCTGTAAGGTTACCCTGGCCGCT > NC_012967/1976848‑1976910
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gTTATAGAAACAGCCTGGTTCATTACAAAATGGTaa < 2:305431/36‑1 (MQ=38)
aaaCAGCCTGGTTCATTACAAAATGGTAATgctgct > 2:155508/1‑36 (MQ=38)
gCCTGGTTCATTACAAAATGGTAATGCTGCTGTAAg < 2:329790/36‑1 (MQ=38)
cTGGTTCATTACAAAATGGTAATGCTGCTGTAAGGt > 1:45467/1‑36 (MQ=38)
cTGGTTCATTACAAAATGGTAATGCTGCTGTAAGGt > 2:699077/1‑36 (MQ=38)
tGGTTCATTACAAAATGGTAATGCTGCTGTAAGGtt > 1:690698/1‑36 (MQ=38)
cATTACAAAATGGTAATGCTGCTGTAAGGTTACCCt > 2:338195/1‑36 (MQ=38)
tACAAAATGGTAATGCTGCTGTAAGGTTACCCTGGc < 2:352755/36‑1 (MQ=38)
tACAAAATGGTAATGCTGCTGTAAGGTTACCCCGGc > 2:753909/1‑36 (MQ=37)
aaaTGGTAATGCTGCTGTAAGGTTACCCTGGCCGCt > 1:631669/1‑36 (MQ=38)
aaaTGGTAATGCTACTGTAAGGTTACCCTGGCCGCt > 1:921569/1‑36 (MQ=37)
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GTTATAGAAACAGCCTGGTTCATTACAAAATTGTAATGCTGCTGTAAGGTTACCCTGGCCGCT > NC_012967/1976848‑1976910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: