Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	2,082,685	G→A	100%	A494V (GCT→GTT)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	2,082,685	0	G	A	100.0%	13.8 / NA	7	A494V (GCT→GTT)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCCTTGCGGTGTCGTGAAGTGCTGCCAGAGCACGTTTTTCTTCCTCCG  >  NC_012967/2082655‑2082703                               |                   acccTTGCGGTGTCGTGAAGTGCTGCCAGAACACGt               >  2:962443/2‑36 (MQ=38)    cTTGCGGTGTATTGAAGTGCTGCCAGAACACGtttt            <  2:202700/36‑1 (MQ=19)        cGGTGTCGTGAAGTGCTGCCAGAACACGTTTttctt        >  1:657407/1‑36 (MQ=38)         ggTGTCGTGAAGTGCTGCCAGAACACGTTTttcttc       >  2:610749/1‑36 (MQ=38)            gtCGTGGAGTGCTGCCAGGACACGTTTTTCTtcctc    <  1:749567/36‑1 (MQ=19)            gtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcctc    >  2:600226/1‑36 (MQ=38)              cGTGAAGTGCTGCCAGAACACGTTTTTCTTCCTCCg  >  1:841856/1‑36 (MQ=38)                               |                   TCCCTTGCGGTGTCGTGAAGTGCTGCCAGAGCACGTTTTTCTTCCTCCG  >  NC_012967/2082655‑2082703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑