Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	2,732,014	C→A	100%	R91L (CGC→CTC)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	2,732,014	0	C	A	100.0%	12.8 / NA	6	R91L (CGC→CTC)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (2/4);  total (2/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGGATAGTGAACTCAGTGGGCAGTTGTGAC  >  NC_012967/2731982‑2732043                                 |                              ttggCGTATTCATGGTGCCGCCTGTGCTCTggagga                            <  1:898426/33‑1 (MQ=37)  ttGCGTATTCATGGTGCCGCCTGTGCTCTGGAGGAt                           <  1:439238/36‑1 (MQ=38)       tATTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGa                      >  1:330075/1‑36 (MQ=38)        aTTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGaa                     <  1:354875/36‑1 (MQ=38)                ggccgccTGTGCTCTGGAGGATAGTGAACTCAGTgg             <  1:507396/35‑1 (MQ=38)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgt     <  1:948204/36‑1 (MQ=39)                           ctctGGAGGATAGTGAACTCAGTGGGCAGTTGTGAc  >  1:20411/1‑36 (MQ=39)                                 |                              TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGGATAGTGAACTCAGTGGGCAGTTGTGAC  >  NC_012967/2731982‑2732043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑