Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	3,045,069	G→T	100%	T312N (ACC→AAC)	sslE ←	lipoprotein metalloprotease SslE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,045,069	0	G	T	100.0%	32.3 / NA	13	T312N (ACC→AAC)	sslE	lipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/6);  total (7/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT  >  NC_012967/3045039‑3045099                               |                               aGTTCAAAGGTATCGATACCAAAGGAGATGTTTTCg                           >  1:225670/1‑36 (MQ=38)   ttCAAAGGTATCGATACCAAAGGAGATGTTATCGcc                         >  2:960075/1‑36 (MQ=37)    tCAAAGGTATCGATACCAAAGGAGATGTTTTCGccc                        <  1:869611/36‑1 (MQ=38)        aGGTAACGATACCAAAGGAGATGTTTTCGCCCCAGc                    <  2:574306/36‑1 (MQ=37)            aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa                >  1:296669/1‑36 (MQ=39)            aTCGATACCAAAGGAGATGTTTTAGCCCCAGCTaaa                >  2:117787/1‑36 (MQ=37)             tCGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAg               <  2:340157/36‑1 (MQ=38)                aTACCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaa            >  1:582933/1‑36 (MQ=38)                aTACCAAAGGAGATGTTTTCGCCCCAGCGAAAGGaa            <  2:622439/36‑1 (MQ=37)                   ccAAAGGAGATGTTTTCGCCCCAGCTAAAGGAAAAt         <  2:366068/36‑1 (MQ=38)                     aaaGGAGATGTTTTCGCCCCAGCGAAAGGAAAATTc       <  2:292035/36‑1 (MQ=37)                         gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa   >  1:465226/1‑36 (MQ=38)                          agaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAt  >  1:400602/1‑36 (MQ=38)                               |                               AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT  >  NC_012967/3045039‑3045099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑