Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,045,069 | G→T | 100% | T312N (ACC→AAC) | sslE ← | lipoprotein metalloprotease SslE |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,045,069 | 0 | G | T | 100.0% | 32.3 / NA | 13 | T312N (ACC→AAC) | sslE | lipoprotein metalloprotease SslE |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (7/6); total (7/6) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT > NC_012967/3045039‑3045099 | aGTTCAAAGGTATCGATACCAAAGGAGATGTTTTCg > 1:225670/1‑36 (MQ=38) ttCAAAGGTATCGATACCAAAGGAGATGTTATCGcc > 2:960075/1‑36 (MQ=37) tCAAAGGTATCGATACCAAAGGAGATGTTTTCGccc < 1:869611/36‑1 (MQ=38) aGGTAACGATACCAAAGGAGATGTTTTCGCCCCAGc < 2:574306/36‑1 (MQ=37) aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa > 1:296669/1‑36 (MQ=39) aTCGATACCAAAGGAGATGTTTTAGCCCCAGCTaaa > 2:117787/1‑36 (MQ=37) tCGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAg < 2:340157/36‑1 (MQ=38) aTACCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaa > 1:582933/1‑36 (MQ=38) aTACCAAAGGAGATGTTTTCGCCCCAGCGAAAGGaa < 2:622439/36‑1 (MQ=37) ccAAAGGAGATGTTTTCGCCCCAGCTAAAGGAAAAt < 2:366068/36‑1 (MQ=38) aaaGGAGATGTTTTCGCCCCAGCGAAAGGAAAATTc < 2:292035/36‑1 (MQ=37) gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa > 1:465226/1‑36 (MQ=38) agaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAt > 1:400602/1‑36 (MQ=38) | AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT > NC_012967/3045039‑3045099 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |