Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	3,045,069	G→T	100%	T312N (ACC→AAC)	sslE ←	lipoprotein metalloprotease SslE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,045,069	0	G	T	100.0%	32.3 / NA	13	T312N (ACC→AAC)	sslE	lipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/6);  total (7/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT > NC_012967/3045039‑3045099 | aGTTCAAAGGTATCGATACCAAAGGAGATGTTTTCg > 1:225670/1‑36 (MQ=38) ttCAAAGGTATCGATACCAAAGGAGATGTTATCGcc > 2:960075/1‑36 (MQ=37) tCAAAGGTATCGATACCAAAGGAGATGTTTTCGccc < 1:869611/36‑1 (MQ=38) aGGTAACGATACCAAAGGAGATGTTTTCGCCCCAGc < 2:574306/36‑1 (MQ=37) aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa > 1:296669/1‑36 (MQ=39) aTCGATACCAAAGGAGATGTTTTAGCCCCAGCTaaa > 2:117787/1‑36 (MQ=37) tCGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAg < 2:340157/36‑1 (MQ=38) aTACCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaa > 1:582933/1‑36 (MQ=38) aTACCAAAGGAGATGTTTTCGCCCCAGCGAAAGGaa < 2:622439/36‑1 (MQ=37) ccAAAGGAGATGTTTTCGCCCCAGCTAAAGGAAAAt < 2:366068/36‑1 (MQ=38) aaaGGAGATGTTTTCGCCCCAGCGAAAGGAAAATTc < 2:292035/36‑1 (MQ=37) gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa > 1:465226/1‑36 (MQ=38) agaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAt > 1:400602/1‑36 (MQ=38) | AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT > NC_012967/3045039‑3045099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑