BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,045,069 G→T 100% T312N (ACC→AAC)  sslE ← lipoprotein metalloprotease SslE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,045,0690GT100.0% 32.3 / NA 13T312N (ACC→AAC) sslElipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/6);  total (7/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT  >  NC_012967/3045039‑3045099
                              |                              
aGTTCAAAGGTATCGATACCAAAGGAGATGTTTTCg                           >  1:225670/1‑36 (MQ=38)
  ttCAAAGGTATCGATACCAAAGGAGATGTTATCGcc                         >  2:960075/1‑36 (MQ=37)
   tCAAAGGTATCGATACCAAAGGAGATGTTTTCGccc                        <  1:869611/36‑1 (MQ=38)
       aGGTAACGATACCAAAGGAGATGTTTTCGCCCCAGc                    <  2:574306/36‑1 (MQ=37)
           aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa                >  1:296669/1‑36 (MQ=39)
           aTCGATACCAAAGGAGATGTTTTAGCCCCAGCTaaa                >  2:117787/1‑36 (MQ=37)
            tCGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAg               <  2:340157/36‑1 (MQ=38)
               aTACCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaa            >  1:582933/1‑36 (MQ=38)
               aTACCAAAGGAGATGTTTTCGCCCCAGCGAAAGGaa            <  2:622439/36‑1 (MQ=37)
                  ccAAAGGAGATGTTTTCGCCCCAGCTAAAGGAAAAt         <  2:366068/36‑1 (MQ=38)
                    aaaGGAGATGTTTTCGCCCCAGCGAAAGGAAAATTc       <  2:292035/36‑1 (MQ=37)
                        gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa   >  1:465226/1‑36 (MQ=38)
                         agaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAt  >  1:400602/1‑36 (MQ=38)
                              |                              
AGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT  >  NC_012967/3045039‑3045099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: