| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_012967 | 3,155,168 | G→A | 100% | G296S (GGC→AGC) | ebgR → | transcriptional regulator EbgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 3,155,168 | 0 | G | A | 100.0% | 27.0 / NA | 10 | G296S (GGC→AGC) | ebgR | transcriptional regulator EbgR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (3/7); total (3/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCG > NC_012967/3155136‑3155198 | tgagcATCCATCCCGAGATGATGGGAAGTCAGAGCg < 1:555669/33‑1 (MQ=25) gcgcATCCATTCCGAGATGATGGGAAGTCAGAGCGt < 2:985086/36‑1 (MQ=38) cATCCATTCCGAGATGATGGGAAGTCAGAGCGTTaa < 1:329052/36‑1 (MQ=39) ttCCGAGATGATGGGAAGTCAGAGCGTTAACCTGgt < 2:388639/36‑1 (MQ=38) agaTGATGGGAAGTCAGAGCGTTAACCTGGTGTATg > 2:277579/1‑36 (MQ=38) atgatgGGAAGTCAGAGCGTTAACCTGGTGTATGaa < 2:482617/36‑1 (MQ=38) atgatgGGAAGTCAGAGCGTTAACCTGGTGTATGaa < 2:877100/36‑1 (MQ=38) atgGGAAGTCAGAGCGTTAACCTGGTGTATGaaaat > 1:650901/1‑35 (MQ=38) gggAAGTCAGAGCGTTAACCTGGTGTATGAAAAAGc > 1:591960/1‑36 (MQ=38) gTCAGAGCGTTAACCTGGTGTATGAAAAAGCCcgcg < 1:10211/36‑1 (MQ=38) | TGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCG > NC_012967/3155136‑3155198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |