BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,200,643 C→A 100% intergenic (+11/‑245) tdcR → / → ECB_RS15790 transcriptional activator TdcR/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,200,6430CA100.0% 20.2 / NA 9intergenic (+11/‑245)tdcR/ECB_RS15790transcriptional activator TdcR/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/3);  total (6/3)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGA  >  NC_012967/3200613‑3200674
                              |                               
aaTGCAATCGTTTTCCATAAATTCTTCTCCACTCAt                            >  2:544789/1‑36 (MQ=37)
       ttgTTTTCCATATATTCTTCTCCACTCATAGGcgac                     <  1:847612/34‑1 (MQ=255)
            ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa                <  1:678329/36‑1 (MQ=39)
            ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa                >  1:64283/1‑36 (MQ=39)
                   aaTTCTTCTCCACTCATAGGCGACGAATAGCAtttt         >  1:776190/1‑36 (MQ=38)
                      tcttctCCACTCATAGGCGACGAATAGCATTTtgtg      <  1:379653/36‑1 (MQ=38)
                        ttctCCACTCATAGGCGACGAATAGCATTTTGTGtt    >  2:172630/1‑36 (MQ=38)
                        ttctCCACTCATAGGCGACGAATAGAATTTTGTGtt    >  1:254762/1‑36 (MQ=25)
                          ctcCACTCATAGGCGACGAATAGCATTTTGTGttca  >  1:273122/1‑34 (MQ=37)
                              |                               
AATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGA  >  NC_012967/3200613‑3200674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: