Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,200,643 | C→A | 100% | intergenic (+11/‑245) | tdcR → / → ECB_RS15790 | transcriptional activator TdcR/hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,200,643 | 0 | C | A | 100.0% | 20.2 / NA | 9 | intergenic (+11/‑245) | tdcR/ECB_RS15790 | transcriptional activator TdcR/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (6/3); total (6/3) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGA > NC_012967/3200613‑3200674 | aaTGCAATCGTTTTCCATAAATTCTTCTCCACTCAt > 2:544789/1‑36 (MQ=37) ttgTTTTCCATATATTCTTCTCCACTCATAGGcgac < 1:847612/34‑1 (MQ=255) ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa < 1:678329/36‑1 (MQ=39) ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa > 1:64283/1‑36 (MQ=39) aaTTCTTCTCCACTCATAGGCGACGAATAGCAtttt > 1:776190/1‑36 (MQ=38) tcttctCCACTCATAGGCGACGAATAGCATTTtgtg < 1:379653/36‑1 (MQ=38) ttctCCACTCATAGGCGACGAATAGCATTTTGTGtt > 2:172630/1‑36 (MQ=38) ttctCCACTCATAGGCGACGAATAGAATTTTGTGtt > 1:254762/1‑36 (MQ=25) ctcCACTCATAGGCGACGAATAGCATTTTGTGttca > 1:273122/1‑34 (MQ=37) | AATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGA > NC_012967/3200613‑3200674 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |