Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	3,248,957	A→T	100%	D764E (GAT→GAA)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,248,957	0	A	T	100.0%	38.0 / NA	14	D764E (GAT→GAA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/8);  total (6/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTG  >  NC_012967/3248925‑3248988                                 |                                aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                              <  1:163156/36‑1 (MQ=39)      gTTATAGATGACGGAGTAGTAACGCAGTTCCAGGCt                         <  1:266355/36‑1 (MQ=38)       ttATAGATGACGGAGTAGTCACGCCGTTCCAGGcct                        >  1:124373/1‑34 (MQ=16)       ttATAGATGACGGAGTAGTAACGCAGTTCCAGGCtt                        <  2:778750/36‑1 (MQ=38)       ttATAGATGACGGAGTAGTAACGCAGTTCCAGGCtt                        <  2:316520/36‑1 (MQ=38)        tataGATGACGGAGTAGTAACGCAGTTCCAGGCttt                       >  1:514290/1‑36 (MQ=38)          taGATGACGGAGTAGTAACGCAGTTCCAGGCTTTcc                     <  1:775410/36‑1 (MQ=38)               gACGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTc                <  2:385514/36‑1 (MQ=38)                aCGGAGTAGTAATGCAGTTCCAGGCTTTCCGCTTCa               >  2:282991/1‑36 (MQ=37)                aCGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCa               <  2:697637/36‑1 (MQ=38)                   gAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCaacc            >  1:53240/1‑34 (MQ=37)                     gtagtaACTCAGTTCCAGGCTTTCCGCTTCAATCAc          <  2:229619/36‑1 (MQ=37)                          aaCGCAGTTCCAGGCTTTCCGCTTCAATCACTTTAc     >  2:888112/1‑36 (MQ=38)                             gCAGTTCCAGGCTTTCCGCTTCAATCACTTTACGTg  >  1:18828/1‑36 (MQ=39)                                 |                                ATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTG  >  NC_012967/3248925‑3248988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑