Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	3,288,092	G→A	100%	R57C (CGT→TGT)	arcB ←	aerobic respiration two‑component sensor histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,288,092	0	G	A	100.0%	27.9 / NA	10	R57C (CGT→TGT)	arcB	aerobic respiration two‑component sensor histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAC  >  NC_012967/3288068‑3288118                         |                           tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                 >  2:671595/1‑36 (MQ=38) tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                 >  1:92353/1‑36 (MQ=38)    gCAAACCAAAGAAGATAGAACAAATAACATCAATGc              <  2:845717/36‑1 (MQ=38)            aagaagATAGAACAAATAACATCAATGCTTTCGAcc      >  1:85792/1‑36 (MQ=38)             agaagaTAGAACAAATAACATCAATGCTTTCGACCt     >  1:928301/1‑36 (MQ=38)             agaagaTAGAACAAATAACATCAATGCTTTCGACCt     >  1:358566/1‑36 (MQ=38)             agaagaTAGAACAAATAACATCAATGCTTTCGACCt     <  2:718547/36‑1 (MQ=38)               aagaTAGAACAAATAACATCAATGCTTTCGACCTGa   <  2:489471/36‑1 (MQ=38)                agaTTGAACAAATAACATCAATGCTTTCGACCTGAc  <  2:309264/36‑1 (MQ=25)                agaTAGAACAAATAACATCAATGCTTTCGACCTGAc  >  1:536369/1‑36 (MQ=38)                         |                           TCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAC  >  NC_012967/3288068‑3288118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑