BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,288,092 G→A 100% R57C (CGT→TGT)  arcB ← aerobic respiration two‑component sensor histidine kinase ArcB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,288,0920GA100.0% 27.9 / NA 10R57C (CGT→TGT) arcBaerobic respiration two‑component sensor histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAC  >  NC_012967/3288068‑3288118
                        |                          
tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                 >  2:671595/1‑36 (MQ=38)
tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                 >  1:92353/1‑36 (MQ=38)
   gCAAACCAAAGAAGATAGAACAAATAACATCAATGc              <  2:845717/36‑1 (MQ=38)
           aagaagATAGAACAAATAACATCAATGCTTTCGAcc      >  1:85792/1‑36 (MQ=38)
            agaagaTAGAACAAATAACATCAATGCTTTCGACCt     >  1:928301/1‑36 (MQ=38)
            agaagaTAGAACAAATAACATCAATGCTTTCGACCt     >  1:358566/1‑36 (MQ=38)
            agaagaTAGAACAAATAACATCAATGCTTTCGACCt     <  2:718547/36‑1 (MQ=38)
              aagaTAGAACAAATAACATCAATGCTTTCGACCTGa   <  2:489471/36‑1 (MQ=38)
               agaTTGAACAAATAACATCAATGCTTTCGACCTGAc  <  2:309264/36‑1 (MQ=25)
               agaTAGAACAAATAACATCAATGCTTTCGACCTGAc  >  1:536369/1‑36 (MQ=38)
                        |                          
TCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAC  >  NC_012967/3288068‑3288118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: