BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 380,188 A→C 100% F239L (TTT→TTG araJ ← MFS transporter AraJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012967380,1880AC100.0% 20.7 / NA 8F239L (TTT→TTGaraJMFS transporter AraJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/5);  total (3/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG  >  NC_012967/380168‑380216
                    |                            
accccAACTAACATCATAATCAAGGTCATCGCCGtt               <  1:504247/34‑1 (MQ=38)
 gCCCAACTAACATCATAATCAAGGTCATCGCCGttt              <  1:433708/36‑1 (MQ=39)
    cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg           >  2:256457/1‑36 (MQ=38)
    cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg           >  2:271947/1‑36 (MQ=38)
     aaCTAACATCATAATCAAGGTCATCGCCGTTTCCGa          <  2:709218/36‑1 (MQ=38)
           catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc    <  2:742045/36‑1 (MQ=38)
           catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc    <  1:34393/36‑1 (MQ=38)
             tcatAATCAAGGTCATCGCCGTTTCCGAAAAACCgg  >  1:285683/1‑36 (MQ=38)
                    |                            
AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG  >  NC_012967/380168‑380216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: