Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	380,188	A→C	100%	F239L (TTT→TTG)	araJ ←	MFS transporter AraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	380,188	0	A	C	100.0%	20.7 / NA	8	F239L (TTT→TTG)	araJ	MFS transporter AraJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/5);  total (3/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG  >  NC_012967/380168‑380216                     |                             accccAACTAACATCATAATCAAGGTCATCGCCGtt               <  1:504247/34‑1 (MQ=38)  gCCCAACTAACATCATAATCAAGGTCATCGCCGttt              <  1:433708/36‑1 (MQ=39)     cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg           >  2:256457/1‑36 (MQ=38)     cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg           >  2:271947/1‑36 (MQ=38)      aaCTAACATCATAATCAAGGTCATCGCCGTTTCCGa          <  2:709218/36‑1 (MQ=38)            catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc    <  2:742045/36‑1 (MQ=38)            catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc    <  1:34393/36‑1 (MQ=38)              tcatAATCAAGGTCATCGCCGTTTCCGAAAAACCgg  >  1:285683/1‑36 (MQ=38)                     |                             AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG  >  NC_012967/380168‑380216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑