Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 380,188 | A→C | 100% | F239L (TTT→TTG) | araJ ← | MFS transporter AraJ |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 380,188 | 0 | A | C | 100.0% | 20.7 / NA | 8 | F239L (TTT→TTG) | araJ | MFS transporter AraJ |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (3/5); total (3/5) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG > NC_012967/380168‑380216 | accccAACTAACATCATAATCAAGGTCATCGCCGtt < 1:504247/34‑1 (MQ=38) gCCCAACTAACATCATAATCAAGGTCATCGCCGttt < 1:433708/36‑1 (MQ=39) cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg > 2:256457/1‑36 (MQ=38) cAACTAACATCATAATCAAGGTCATCGCCGTTTCCg > 2:271947/1‑36 (MQ=38) aaCTAACATCATAATCAAGGTCATCGCCGTTTCCGa < 2:709218/36‑1 (MQ=38) catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc < 2:742045/36‑1 (MQ=38) catcatAATCAAGGTCATCGCCGTTTCCGAAAAAcc < 1:34393/36‑1 (MQ=38) tcatAATCAAGGTCATCGCCGTTTCCGAAAAACCgg > 1:285683/1‑36 (MQ=38) | AGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGG > NC_012967/380168‑380216 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |