Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,339,158 | A→C | 100% | intergenic (+22/‑4) | dusB → / → fis | tRNA dihydrouridine synthase DusB/DNA‑binding transcriptional regulator Fis |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,339,158 | 0 | A | C | 100.0% | 44.3 / NA | 16 | intergenic (+22/‑4) | dusB/fis | tRNA dihydrouridine synthase DusB/DNA‑binding transcriptional regulator Fis |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (5/11); total (5/11) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTC > NC_012967/3339128‑3339184 | tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt < 1:768788/36‑1 (MQ=38) tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt > 1:89192/1‑36 (MQ=38) tGCGTAAACAGAAATAAAGAGCTGACAGCACTATGt < 1:339432/36‑1 (MQ=39) acGTAAACAGAAATAAAGAGCTGACAGCACTATGtt < 1:919511/35‑1 (MQ=38) cGTAAACAGAAATAAAGAGCTGACAGCACCATGtcc > 1:366208/1‑34 (MQ=25) gTAAACAGAAATAAAGAGCTGACAGCACTATGTTCg < 2:133926/36‑1 (MQ=39) tAAACAGAAATAAAGAGCTGACAGCACTATGTTCGa < 2:413743/36‑1 (MQ=39) aaaCAGAAATAAAGAGCTGACAGCACTATGTTCGaa < 2:152870/36‑1 (MQ=39) aaaCAGAAATAAAGAACTCACAGCACTATGTTCGaa < 1:466994/36‑1 (MQ=16) cAGAAATAAAAAACTGACAGCACTATGTTCGaacaa < 1:551724/36‑1 (MQ=19) aGAAATAAAGAGCTGACAGCACTATGTTCGaacaac > 1:194947/1‑36 (MQ=39) aTAAAGAGCTGACAGCACTATGTTCGAACAACGCGt < 1:256298/36‑1 (MQ=39) aaaGTGCTGACAGCACTATGTTCGAACAACGCGTaa < 1:713281/36‑1 (MQ=37) aaaGGGCTGACAGCACTATGTTCGAACAACGCGTaa < 1:517003/36‑1 (MQ=37) gagCTGACAGCACTATGTTCGAACAACGCGTAAAtt > 2:166040/1‑36 (MQ=39) agCTGACAGCACTATGTTCGAACAACGCGTAAATTc > 1:800911/1‑36 (MQ=39) | TTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTC > NC_012967/3339128‑3339184 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |