BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,369,432 T→C 100% D50G (GAC→GGC)  rpsD ← 30S ribosomal protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,369,4320TC100.0% 32.5 / NA 12D50G (GAC→GGC) rpsD30S ribosomal protein S4
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/3);  total (9/3)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCG  >  NC_012967/3369404‑3369458
                            |                          
ttGCTTTTCACGCAACTGCACACCATAGCcagacag                     >  1:369809/1‑36 (MQ=38)
  gCTTTTCACGCAACTGCACACCATAGCCagacagac                   >  1:494902/1‑36 (MQ=38)
         aCGCAACTGCACACCATAGCCAGACAGACGCGGttt            >  1:858144/1‑36 (MQ=38)
          cGCAACTGCACACCATAGCCAGACAGACGCGGTTTa           >  2:911930/1‑36 (MQ=38)
             aaCTGCACACCATAGCCAGACAGACGCGGTTTAcgc        <  2:824304/36‑1 (MQ=38)
              aCTGCACACCATTGCCAGTCAGACGCGGTTTAcgcg       >  2:624722/1‑36 (MQ=16)
              aCTGCACACCATAGCCAGACAGACGCGGTTTAcgcg       >  1:374614/1‑36 (MQ=38)
               cTGCACACCATAGCCAGACAGACGCGGTTTACacgc      >  2:617292/1‑36 (MQ=37)
                tGCACACCATAGCCAGACAGACGCGGTTTACGCGCa     >  2:86697/1‑36 (MQ=37)
                 gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc    >  1:122656/1‑36 (MQ=37)
                 gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc    <  2:4899/36‑1 (MQ=37)
                   acacCAGAGCCAGACAGACGCGGTTTACGCGCACCg  <  1:42763/36‑1 (MQ=25)
                            |                          
TTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCG  >  NC_012967/3369404‑3369458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: