Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,369,432 | T→C | 100% | D50G (GAC→GGC) | rpsD ← | 30S ribosomal protein S4 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,369,432 | 0 | T | C | 100.0% | 32.5 / NA | 12 | D50G (GAC→GGC) | rpsD | 30S ribosomal protein S4 |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/3); total (9/3) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCG > NC_012967/3369404‑3369458 | ttGCTTTTCACGCAACTGCACACCATAGCcagacag > 1:369809/1‑36 (MQ=38) gCTTTTCACGCAACTGCACACCATAGCCagacagac > 1:494902/1‑36 (MQ=38) aCGCAACTGCACACCATAGCCAGACAGACGCGGttt > 1:858144/1‑36 (MQ=38) cGCAACTGCACACCATAGCCAGACAGACGCGGTTTa > 2:911930/1‑36 (MQ=38) aaCTGCACACCATAGCCAGACAGACGCGGTTTAcgc < 2:824304/36‑1 (MQ=38) aCTGCACACCATTGCCAGTCAGACGCGGTTTAcgcg > 2:624722/1‑36 (MQ=16) aCTGCACACCATAGCCAGACAGACGCGGTTTAcgcg > 1:374614/1‑36 (MQ=38) cTGCACACCATAGCCAGACAGACGCGGTTTACacgc > 2:617292/1‑36 (MQ=37) tGCACACCATAGCCAGACAGACGCGGTTTACGCGCa > 2:86697/1‑36 (MQ=37) gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc > 1:122656/1‑36 (MQ=37) gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc < 2:4899/36‑1 (MQ=37) acacCAGAGCCAGACAGACGCGGTTTACGCGCACCg < 1:42763/36‑1 (MQ=25) | TTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCG > NC_012967/3369404‑3369458 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |