BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,370,027 T→A 100% K117M (AAG→ATG)  rpsM ← 30S ribosomal protein S13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,370,0270TA100.0% 33.8 / NA 12K117M (AAG→ATG) rpsM30S ribosomal protein S13
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/8);  total (4/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTA  >  NC_012967/3370000‑3370056
                           |                             
ccATTATTCAATCACCCCGATTATTTCATGATCGGt                       <  1:16553/36‑1 (MQ=38)
ccATTATTCAATCACCCCGATTATTTCATGATCGGt                       <  2:464607/36‑1 (MQ=38)
  attattCAATCACCCCGATTATTTCATGATCGGttt                     >  1:427871/1‑36 (MQ=38)
     attCAATCACCCCGATTATTTCATGATCGGTTTgcg                  <  1:677851/36‑1 (MQ=39)
     attCAATCACCCCGATTATTTCATGATCGGTTTgcg                  <  2:175799/36‑1 (MQ=39)
      ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc                 <  1:698054/36‑1 (MQ=39)
        cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg               >  1:702598/1‑36 (MQ=39)
                 cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg      <  2:740499/36‑1 (MQ=39)
                 cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg      <  1:26093/36‑1 (MQ=39)
                  gATTATTTCATGATCGGTTTGCGCGGACCCTTACgg     <  2:402388/36‑1 (MQ=39)
                   attattTCATGATCGGTTTGCGCGGACCCTTACggg    >  2:458112/1‑36 (MQ=39)
                     tattTCATGATCGGTTTGCGCGGACCCTTACGGGTa  >  2:946237/1‑36 (MQ=39)
                           |                             
CCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTA  >  NC_012967/3370000‑3370056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: