Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,370,027 | T→A | 100% | K117M (AAG→ATG) | rpsM ← | 30S ribosomal protein S13 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,370,027 | 0 | T | A | 100.0% | 33.8 / NA | 12 | K117M (AAG→ATG) | rpsM | 30S ribosomal protein S13 |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (4/8); total (4/8) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTA > NC_012967/3370000‑3370056 | ccATTATTCAATCACCCCGATTATTTCATGATCGGt < 1:16553/36‑1 (MQ=38) ccATTATTCAATCACCCCGATTATTTCATGATCGGt < 2:464607/36‑1 (MQ=38) attattCAATCACCCCGATTATTTCATGATCGGttt > 1:427871/1‑36 (MQ=38) attCAATCACCCCGATTATTTCATGATCGGTTTgcg < 1:677851/36‑1 (MQ=39) attCAATCACCCCGATTATTTCATGATCGGTTTgcg < 2:175799/36‑1 (MQ=39) ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc < 1:698054/36‑1 (MQ=39) cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg > 1:702598/1‑36 (MQ=39) cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg < 2:740499/36‑1 (MQ=39) cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg < 1:26093/36‑1 (MQ=39) gATTATTTCATGATCGGTTTGCGCGGACCCTTACgg < 2:402388/36‑1 (MQ=39) attattTCATGATCGGTTTGCGCGGACCCTTACggg > 2:458112/1‑36 (MQ=39) tattTCATGATCGGTTTGCGCGGACCCTTACGGGTa > 2:946237/1‑36 (MQ=39) | CCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTA > NC_012967/3370000‑3370056 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |