BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 3,483,047 C→A 100% R455S (CGC→AGC)  malT → HTH‑type transcriptional regulator MalT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129673,483,0470CA100.0% 23.6 / ‑6.7 10R455S (CGC→AGC) malTHTH‑type transcriptional regulator MalT
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (6/3);  total (7/3)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATC  >  NC_012967/3483026‑3483077
                     |                              
cACGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCg                  <  1:563827/36‑1 (MQ=39)
 aCGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGa                 >  2:511103/1‑36 (MQ=39)
  cGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGAt                <  2:641408/36‑1 (MQ=39)
   gCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGAtt               >  1:46547/1‑36 (MQ=39)
    cAGAATTTAACGCTCTGAGCGCCCAGGTGGCGATTa              <  1:967725/36‑1 (MQ=39)
       aaTTTAACGCTCTGAGCGCCCAGGTGGCGATTAACg           >  1:471482/1‑36 (MQ=38)
               gCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAAt   >  2:749977/1‑36 (MQ=255)
               gCTCTGAGCGCCCAGGTGGCGATTAACGATGGTAAt   >  2:519355/1‑36 (MQ=38)
                ctctGAGCGCCCAGGTGGCGATTAACGATGGTCATc  >  2:215291/1‑36 (MQ=37)
                ctctGAGCGCCCAGGTGGCGATTAACGATGGTAATc  >  1:931370/1‑36 (MQ=38)
                     |                              
CACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATC  >  NC_012967/3483026‑3483077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: