Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 3,483,047 | C→A | 100% | R455S (CGC→AGC) | malT → | HTH‑type transcriptional regulator MalT |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 3,483,047 | 0 | C | A | 100.0% | 23.6 / ‑6.7 | 10 | R455S (CGC→AGC) | malT | HTH‑type transcriptional regulator MalT |
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (6/3); total (7/3) | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATC > NC_012967/3483026‑3483077 | cACGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCg < 1:563827/36‑1 (MQ=39) aCGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGa > 2:511103/1‑36 (MQ=39) cGCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGAt < 2:641408/36‑1 (MQ=39) gCAGAATTTAACGCTCTGAGCGCCCAGGTGGCGAtt > 1:46547/1‑36 (MQ=39) cAGAATTTAACGCTCTGAGCGCCCAGGTGGCGATTa < 1:967725/36‑1 (MQ=39) aaTTTAACGCTCTGAGCGCCCAGGTGGCGATTAACg > 1:471482/1‑36 (MQ=38) gCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAAt > 2:749977/1‑36 (MQ=255) gCTCTGAGCGCCCAGGTGGCGATTAACGATGGTAAt > 2:519355/1‑36 (MQ=38) ctctGAGCGCCCAGGTGGCGATTAACGATGGTCATc > 2:215291/1‑36 (MQ=37) ctctGAGCGCCCAGGTGGCGATTAACGATGGTAATc > 1:931370/1‑36 (MQ=38) | CACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATC > NC_012967/3483026‑3483077 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |