Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	3,490,112	C→T	100%	A105T (GCG→ACG)	glpE ←	thiosulfate sulfurtransferase GlpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,490,112	0	C	T	100.0%	42.1 / NA	16	A105T (GCG→ACG)	glpE	thiosulfate sulfurtransferase GlpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/6);  total (10/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGTCGCTGCCAGGCT  >  NC_012967/3490082‑3490143                               |                                gggACAGTATAAAGCGTTACGCGCCGTACGTCACct                            <  1:745871/36‑1 (MQ=39)      aGTATAGAACGTTACGCGGCGTACGTCACCTCTGCg                       <  1:877007/36‑1 (MQ=12)        tataAAGCGTTACGCGCCGTACGTCACCTCTGCggg                     <  1:830706/36‑1 (MQ=39)              gCGTTACGCGCCGTACGTCACCTCTGCGGGAAACTg               >  1:281499/1‑36 (MQ=39)                gTTACGCGCCGTACGTCACCTCTGCGGGAAACTGTc             <  2:680625/36‑1 (MQ=39)                gTTACGCGCCGTACGTCACCTCTGCGGGAAACTGTc             >  1:418318/1‑36 (MQ=39)                 ttACGCGCCGTACGTCACCTCTGCGGGAAACTGTCg            >  1:673623/1‑36 (MQ=39)                  tACGCGCCGTACGTCACCTCTGCGGGAAACTGTCGc           >  2:650616/1‑36 (MQ=39)                   aCGCGCCGTACGTCACCTCTGCGGGAAACTGTCGCt          >  2:9526/1‑36 (MQ=39)                    cgcgCCGTACGTCACCTCTCCGGGAAACTCTCGcac         >  1:977794/1‑34 (MQ=16)                       gcCGTACGTCGCTTCTGCGGGAAACTGTCGCTGCCa      <  1:529802/36‑1 (MQ=16)                       gcCGTACGTCACCTCTGCGGGAAACTGTCGCTGctt      >  1:660059/1‑34 (MQ=37)                          gTACGTCACCTCTGCGGGAAACTGTCGCTGCCAGGc   >  1:492415/1‑36 (MQ=38)                          gTACGTCACCTCTGCGGGAAACTGTCGCTGCCAGGc   >  2:842592/1‑36 (MQ=38)                          gTACGTCACCTCTGCGGGAAACTGTCGCTGCCAGGc   <  1:606757/36‑1 (MQ=38)                           tACGTCACCTCTGCGGGAAACTGTCGCTGCCAAGCt  >  2:928302/1‑36 (MQ=25)                               |                                GGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGTCGCTGCCAGGCT  >  NC_012967/3490082‑3490143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑