Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	4,100,655	C→T	100%	M192I (ATG→ATA)	hslU ←	HslU‑‑HslV peptidase ATPase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	4,100,655	0	C	T	100.0%	19.6 / NA	9	M192I (ATG→ATA)	hslU	HslU‑‑HslV peptidase ATPase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTGCAGCTGGCTGGTCATCTCTTCCATGCCCGGAGGAGCCATAATTTC  >  NC_012967/4100630‑4100678                          |                        aCTGCAGCTGGCTGGTCCTCTCTTCTATGCCCggag               >  2:738610/1‑36 (MQ=37) aCTGCAGCTGGCGGGTCATCACTTCTATGCCCggag               >  2:289620/1‑36 (MQ=16)    gCAGCTGGCTGGTCATCTCTTCTATGCCCGgaggag            <  2:106450/36‑1 (MQ=38)      aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc          <  2:829130/36‑1 (MQ=38)      aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc          >  2:444456/1‑36 (MQ=38)       gctggttgGTCATCTCTTCTATGCCCGGAGGAGCCa         >  1:400876/1‑36 (MQ=37)         tggctggTCATCTCTTGTATGCCGGGAGGAGCCATa       <  1:82341/36‑1 (MQ=19)             ttttCATCTCTTCTATTCCGGGAGGAGCCATAAttt   <  1:428870/33‑1 (MQ=16)              ggTCATCTCTTCTATGCCCGGAGGAGCCATAATTTc  >  2:406998/1‑36 (MQ=39)                          |                        ACTGCAGCTGGCTGGTCATCTCTTCCATGCCCGGAGGAGCCATAATTTC  >  NC_012967/4100630‑4100678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑