Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 4,201,911 | C→T | 100% | A217T (GCG→ACG) | iclR ← | glyoxylate bypass operon transcriptional repressor IclR |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 4,201,911 | 0 | C | T | 96.1% | 45.9 / ‑6.1 | 18 | A217T (GCG→ACG) | iclR | glyoxylate bypass operon transcriptional repressor IclR |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/8); minor base A (0/1); total (9/9) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAAC > NC_012967/4201879‑4201937 | aaTACACGCTGCAAGGCAACGTAGCCCCCGCGTATg > 2:225789/1‑36 (MQ=37) aTACACGCTGCAAGGCAACGTAGCCCCAGCGTATGt > 2:440666/1‑36 (MQ=38) tACACGCTGCAAGGCAACGTAGCCCCAGCGTATGtt < 2:388340/36‑1 (MQ=38) acacGCTGCAAGGCAACGTAGCCCCAGCGTATGtta > 1:539173/1‑35 (MQ=38) cacGCTGCAAGGCAACGTAGCCCCAGCGTATGTTcc > 1:322309/1‑36 (MQ=38) cTGCAAGGCAACGTAGCCCCAGCGTATGTTCCTCAt > 1:863483/1‑36 (MQ=38) gaaaGGCAACGTAGCCCCAGCGTATGTTCCTCATCg < 1:118627/34‑1 (MQ=37) gCAAGGCAACGTAGCCCCAGCGTATGTTCCTCATCg > 1:813299/1‑36 (MQ=38) aaGGCAACGTAGCCCCAGCGTATGTTCCTCATCGTc < 1:837876/36‑1 (MQ=38) aaGGCAACGTAGCCCCAGCGTATGTTCCTCATCGTc > 2:331397/1‑36 (MQ=38) atgCAACGTAGCCCCAGCGTATGTTCCTCATCGTCa < 2:773282/34‑1 (MQ=37) aGGCAACGTAGCCCCAGCGTATGTTCCTCATCGTCa < 2:622822/36‑1 (MQ=38) gaaaCGAAGCCCCAGCGAATGTTCCTCATCGTCaaa < 2:604152/34‑1 (MQ=25) aaCGTAGCCCCAGCGTATGTTCCTCATCGTCATATg > 2:689957/1‑36 (MQ=37) aaCGTAGCCCCAGCGTATGTTCCTCATCGTCAAATg < 1:134193/36‑1 (MQ=38) tAGCCCCAGCGTATGTTCCTCATCGTCAAATGAATa < 2:829144/36‑1 (MQ=38) gCCCCAGCGTATGTTCCTCATCGTCAAATGAATAAc < 1:393560/36‑1 (MQ=38) gCCCCAGCGTATGTTCCTCATCGTCAAATGAATAAc > 2:904714/1‑36 (MQ=38) | AATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAAC > NC_012967/4201879‑4201937 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |