| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_012967 | 4,616,396 | G→A | 100% | G290S (GGC→AGC) | nadR → | multifunctional transcriptional regulator/nicotinamide‑nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 4,616,396 | 0 | G | A | 100.0% | 51.9 / NA | 18 | G290S (GGC→AGC) | nadR | multifunctional transcriptional regulator/nicotinamide‑nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/4); total (14/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAA > NC_012967/4616365‑4616427 | gCAGTATTCCGATTACGATAAAATCGCGCTGAGCCa < 1:698032/36‑1 (MQ=38) gTATTCCGATTACGATAAAATCGCGCTGAGCCACGc < 2:21550/36‑1 (MQ=38) ccGATTACGATAAAATCGCGCTGAGCCACCCACAAt > 2:667936/1‑36 (MQ=25) aTTACGATAAAATCGCGCTGAGCCACGCACAATACa > 1:140752/1‑36 (MQ=38) ttACGATAAAATCGCGCTGAGCCACGCATAATACAt > 2:791104/1‑36 (MQ=25) tACGATAAAATCGCGCTGAGCCACGCACAATACAtt < 1:186046/36‑1 (MQ=38) aCGATAAAATCGCGCTGAGCTACGCACAATACATTg > 1:987676/1‑36 (MQ=25) aCGATAAAATCGCGCTGAGCCACGCACAATACATTg < 1:990717/36‑1 (MQ=38) gATAAAATCGCGCTGAGCCACGCACAATACATTGAt > 1:229018/1‑36 (MQ=38) aTAAAATCGCGCTGAGCCACGCACAATACATTGAtt > 1:115898/1‑36 (MQ=38) aTAAAATCGCGCTGAGCCACGCACAATACATTGAtt > 1:516929/1‑36 (MQ=38) aaaaTCGCGCTGAGCCACGCACAATACATTGAtttt > 2:395206/1‑36 (MQ=38) aaaaTCGCGCTGAGCCACGCACAATACATTGAtttt > 1:210263/1‑36 (MQ=38) aaTCGCGCTGAGCCACGCACAATACATTGTTTTTgt > 1:736659/1‑35 (MQ=25) cgcgCTGAGCCACGCACAATACATTGATTTTGCAGt > 2:107900/1‑36 (MQ=38) gcgcTGAGCCACGCACAATACATTGATTTTGCtgtg > 2:264000/1‑36 (MQ=37) gcTGAGCCACGCCCAATACATTGATTTTGCAGTGaa > 2:911917/1‑36 (MQ=37) gcTGAGCCACGCACAATACATTGATTTTGCAGTGaa > 1:972411/1‑36 (MQ=38) | GCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAA > NC_012967/4616365‑4616427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |