Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 649,391 | T→A | 100% | I471F (ATC→TTC) | mrdA ← | peptidoglycan DD‑transpeptidase MrdA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 649,391 | 0 | T | A | 100.0% | 37.4 / NA | 14 | I471F (ATC→TTC) | mrdA | peptidoglycan DD‑transpeptidase MrdA |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (7/7); total (7/7) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGG > NC_012967/649362‑649420 | aaCCTTCACGATACCGTCATTAATCAGGAACATCAg < 1:235356/36‑1 (MQ=37) aaCCTTCACGATACCGTCACTAATCAGGAacatcaa > 1:451453/1‑35 (MQ=25) cTTCACGATACCGTCATTAATCAGGAACATCAGTGc < 2:543819/36‑1 (MQ=37) ttCACGATACCGTCATTAATCAGGAACATCAGTGcc < 1:570901/36‑1 (MQ=37) ttCACGATACCGTCATTAATCAGGAACATCAGTGcc > 1:900870/1‑36 (MQ=37) cACGTTACCGTCATTAATCAGGAACATCAGTGCCtt < 1:919479/36‑1 (MQ=25) cACGATACCGTCATTAATCAGGAACATCAGTGCCtt < 1:138512/36‑1 (MQ=37) cACGATACCGTCATTAATCAGGAACATCAGCGCCtt > 2:824486/1‑36 (MQ=21) cGATATCGTCATTAATCAGGAACATCAGTGCCTTAc < 2:727904/36‑1 (MQ=25) cGATACCGTCATTAGTCAGGAACATCAGTGCCTTAc > 1:465473/1‑36 (MQ=37) cGATACCGTCATTAATCCGGAACATCAGTGCCttcc > 1:647247/1‑34 (MQ=25) gATACCGTCATTAATCAGGAACATCAGTGCCTTACt < 1:269849/36‑1 (MQ=37) ccGTCATTAATCAGGAACATCAGTGCCTTACTCATc > 2:806664/1‑36 (MQ=38) aaTCAGGAACATCAGTGCCTTACTCATCTCGATTgg > 2:737523/1‑36 (MQ=37) tCAGGAACATCAGTGCCTTACTCATCTGGATTgggg > 1:279810/1‑36 (MQ=38) | AACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGG > NC_012967/649362‑649420 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |