| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_012967 | 668,787 | A→C | 100% | Y65D (TAC→GAC) | gltI ← | glutamate/aspartate ABC transporter substrate‑binding protein GltI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 668,787 | 0 | A | C | 100.0% | 25.5 / NA | 10 | Y65D (TAC→GAC) | gltI | glutamate/aspartate ABC transporter substrate‑binding protein GltI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (4/6); total (4/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATA > NC_012967/668762‑668817 | aTGGCGTTGGAGTAATCCTGCGAGTCACCCACCACt > 1:849476/1‑36 (MQ=39) ggCGTTGGAGAAATCCTGCGAGTCACCCACCACttt < 1:986120/36‑1 (MQ=37) cGTTGGAGTAATCCTGCGAGTCACCCACCACttttt > 2:119935/1‑36 (MQ=38) tGGAGTAATCCTGCGAGTCACCCACCACTTTTTGCt < 1:239504/36‑1 (MQ=38) tGGAGTAATCCTGCGAGTCACCCACCACTTTTTGCt < 1:857985/36‑1 (MQ=38) gTAATCCTGCGAGTCACCCACCACTTTTTGCTGAtt > 1:951642/1‑36 (MQ=37) tAATCCTGCGAGTCACCCACCACTTTTTGCTGATTg > 2:921507/1‑36 (MQ=25) acccTGCGAGTCACCCACCACTTTTTGCTGATTGTc < 1:986382/34‑1 (MQ=21) cGAGTCACCCACCTCTTTTTGCTGATGGTCGtaata < 2:83102/36‑1 (MQ=16) cGAGTCACCCACCACTTTTTGCTGATTGTCGtaata < 1:937446/36‑1 (MQ=25) | ATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATA > NC_012967/668762‑668817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |