| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_012967 | 683,496 | A→C | 100% | V65G (GTT→GGT) | nagC ← | DNA‑binding transcriptional regulator NagC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 683,496 | 0 | A | C | 100.0% | 20.0 / NA | 10 | V65G (GTT→GGT) | nagC | DNA‑binding transcriptional regulator NagC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (6/4); total (6/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGCGGCCCCCAGTGGAGGCCTGCTGATCAACCTCTTTGATCAGCCCGCGTTCGATAAGCTG > NC_012967/683465‑683527 | gcggcgGCCCCCCGTGGAGGGCTGCTGATCACCctc > 1:651462/1‑36 (MQ=19)gcggccGCCCCCAGTGGAGGCCTGCTGATCACCctc < 2:907789/36‑1 (MQ=25) ccccGGTGGAGGCCTGCTGATCACCCTCTTTGATCa < 2:849365/36‑1 (MQ=25) cccAGTGGAGGCCTGCTGATCACCCTCTTTGATCAg > 1:322723/1‑36 (MQ=38) gTGGAGGACTGCTGATCACCCTCTTTGATCAGCCcg > 1:208724/1‑36 (MQ=37) ctgctgGTCACCGTCTGTGATCAGCCCGCGTTCGAt < 1:95532/36‑1 (MQ=12) tgctgATCACCCTCTTTGATCAGCCCGCGTTCGATa > 1:989125/1‑36 (MQ=38) gATCACCCTCTTTGATCAGCCCGCGTTCGATATGCt > 2:344707/1‑36 (MQ=37) gATCACCCTCTTTGATCAGCCCGCGTTCGATAAGCt > 1:476776/1‑36 (MQ=38) aTCACCCTCTTTGATCAGCCCGCGTTCGATAAGCTg < 2:320655/36‑1 (MQ=38) | GCGGCGGCCCCCAGTGGAGGCCTGCTGATCAACCTCTTTGATCAGCCCGCGTTCGATAAGCTG > NC_012967/683465‑683527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |