Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	910,316	T→C	100%	intergenic (+176/‑175)	ybjN → / → potF	YbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	910,316	0	T	C	100.0%	43.8 / NA	15	intergenic (+176/‑175)	ybjN/potF	YbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/12);  total (3/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT  >  NC_012967/910289‑910347                            |                                aaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAcc                         >  2:550088/1‑36 (MQ=39)    aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                      <  2:94375/36‑1 (MQ=39)    aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                      <  2:493976/36‑1 (MQ=39)     ccGTTTTTTAATCCGATCTATAGCCTCAAACCCTgg                     <  1:855243/36‑1 (MQ=37)       gTTTTTTAATCCGAGTTATAGCCTCAAACCCTGGCt                   <  1:370193/36‑1 (MQ=37)        ttttttAATCCGAGCTATAGCCTCAAACCCTGGCTa                  >  1:603025/1‑36 (MQ=39)          ttttAATCCGAGCTATAGCCTCAAACCCTGGCTaaa                <  1:148075/36‑1 (MQ=39)           ttaaaTCCGAGCTATAGCCTCAAACCCTGGCTAAAg               <  2:375400/33‑1 (MQ=37)                  cGAGCTTTAGCCTCAAACCCTGGCTAAAGTTATTCt        <  1:926813/36‑1 (MQ=37)                  cGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCt        <  1:942100/36‑1 (MQ=38)                   gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt       >  1:306611/1‑36 (MQ=38)                     gCTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGc     <  1:786975/36‑1 (MQ=38)                     gCTATAGCCCCAAACCCTGGCTAAAGTTATTCTTGc     <  1:986648/36‑1 (MQ=37)                      cTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGCg    <  1:679623/36‑1 (MQ=38)                        ataGCCTCAAACCCTGGCTAAAGTTATTCTTGCGAt  <  1:982278/36‑1 (MQ=38)                            |                                AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT  >  NC_012967/910289‑910347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑