Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 910,316 | T→C | 100% | intergenic (+176/‑175) | ybjN → / → potF | YbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 910,316 | 0 | T | C | 100.0% | 43.8 / NA | 15 | intergenic (+176/‑175) | ybjN/potF | YbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (3/12); total (3/12) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT > NC_012967/910289‑910347 | aaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAcc > 2:550088/1‑36 (MQ=39) aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg < 2:94375/36‑1 (MQ=39) aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg < 2:493976/36‑1 (MQ=39) ccGTTTTTTAATCCGATCTATAGCCTCAAACCCTgg < 1:855243/36‑1 (MQ=37) gTTTTTTAATCCGAGTTATAGCCTCAAACCCTGGCt < 1:370193/36‑1 (MQ=37) ttttttAATCCGAGCTATAGCCTCAAACCCTGGCTa > 1:603025/1‑36 (MQ=39) ttttAATCCGAGCTATAGCCTCAAACCCTGGCTaaa < 1:148075/36‑1 (MQ=39) ttaaaTCCGAGCTATAGCCTCAAACCCTGGCTAAAg < 2:375400/33‑1 (MQ=37) cGAGCTTTAGCCTCAAACCCTGGCTAAAGTTATTCt < 1:926813/36‑1 (MQ=37) cGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCt < 1:942100/36‑1 (MQ=38) gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt > 1:306611/1‑36 (MQ=38) gCTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGc < 1:786975/36‑1 (MQ=38) gCTATAGCCCCAAACCCTGGCTAAAGTTATTCTTGc < 1:986648/36‑1 (MQ=37) cTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGCg < 1:679623/36‑1 (MQ=38) ataGCCTCAAACCCTGGCTAAAGTTATTCTTGCGAt < 1:982278/36‑1 (MQ=38) | AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT > NC_012967/910289‑910347 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |