BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 910,316 T→C 100% intergenic (+176/‑175) ybjN → / → potF YbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012967910,3160TC100.0% 43.8 / NA 15intergenic (+176/‑175)ybjN/potFYbjN domain‑containing protein/spermidine/putrescine ABC transporter substrate‑binding protein PotF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/12);  total (3/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT  >  NC_012967/910289‑910347
                           |                               
aaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAcc                         >  2:550088/1‑36 (MQ=39)
   aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                      <  2:94375/36‑1 (MQ=39)
   aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                      <  2:493976/36‑1 (MQ=39)
    ccGTTTTTTAATCCGATCTATAGCCTCAAACCCTgg                     <  1:855243/36‑1 (MQ=37)
      gTTTTTTAATCCGAGTTATAGCCTCAAACCCTGGCt                   <  1:370193/36‑1 (MQ=37)
       ttttttAATCCGAGCTATAGCCTCAAACCCTGGCTa                  >  1:603025/1‑36 (MQ=39)
         ttttAATCCGAGCTATAGCCTCAAACCCTGGCTaaa                <  1:148075/36‑1 (MQ=39)
          ttaaaTCCGAGCTATAGCCTCAAACCCTGGCTAAAg               <  2:375400/33‑1 (MQ=37)
                 cGAGCTTTAGCCTCAAACCCTGGCTAAAGTTATTCt        <  1:926813/36‑1 (MQ=37)
                 cGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCt        <  1:942100/36‑1 (MQ=38)
                  gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt       >  1:306611/1‑36 (MQ=38)
                    gCTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGc     <  1:786975/36‑1 (MQ=38)
                    gCTATAGCCCCAAACCCTGGCTAAAGTTATTCTTGc     <  1:986648/36‑1 (MQ=37)
                     cTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGCg    <  1:679623/36‑1 (MQ=38)
                       ataGCCTCAAACCCTGGCTAAAGTTATTCTTGCGAt  <  1:982278/36‑1 (MQ=38)
                           |                               
AATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT  >  NC_012967/910289‑910347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: