BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012967 1,248,380 A→C 100% D295E (GAT→GAG)  dhaM ← dihydroxyacetone kinase subunit DhaM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129671,248,3800AC89.4% 16.7 / ‑5.0 9D295E (GAT→GAG) dhaMdihydroxyacetone kinase subunit DhaM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (3/5);  minor base T (1/0);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT  >  NC_012967/1248348‑1248410
                                |                              
cTGGCTTCTGCTTTCGCTGTTAACGTCATCAGctct                             >  1:490537/1‑36 (MQ=38)
       cTGCTTTCGCTGTTAACGTCATCAGTTCtaataatg                      >  1:590347/1‑34 (MQ=37)
       cTGCTTTAGATGTTAACGTCATCAGCTCTAATAACg                      <  1:237367/36‑1 (MQ=19)
        ttcTTTCGCTGTTAACGTCATCAGCTCTAATAACGt                     <  1:390440/34‑1 (MQ=37)
            ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg                 >  1:225033/1‑36 (MQ=38)
            ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg                 <  1:749685/36‑1 (MQ=38)
               gCTGTTAACGTCATCAGCTCTAATAACGCGAAGTCa              >  2:887531/1‑36 (MQ=37)
                  gTTAACGTCATCAGCTCTAATAACGTGAAGTCAATa           <  2:974576/36‑1 (MQ=38)
                      aCGTCATCAGCTCTAATAACGTGAAGTCAATAGCCt       <  1:386732/36‑1 (MQ=255)
                           atcaGCTCTAATAACGTGAAGTCAATAGCCTGGCGt  >  1:757064/1‑36 (MQ=38)
                                |                              
CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT  >  NC_012967/1248348‑1248410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑