Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_012967 | 1,248,380 | A→C | 100% | D295E (GAT→GAG) | dhaM ← | dihydroxyacetone kinase subunit DhaM |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_012967 | 1,248,380 | 0 | A | C | 89.4% | 16.7 / ‑5.0 | 9 | D295E (GAT→GAG) | dhaM | dihydroxyacetone kinase subunit DhaM |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (3/5); minor base T (1/0); total (4/5) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT > NC_012967/1248348‑1248410 | cTGGCTTCTGCTTTCGCTGTTAACGTCATCAGctct > 1:490537/1‑36 (MQ=38) cTGCTTTCGCTGTTAACGTCATCAGTTCtaataatg > 1:590347/1‑34 (MQ=37) cTGCTTTAGATGTTAACGTCATCAGCTCTAATAACg < 1:237367/36‑1 (MQ=19) ttcTTTCGCTGTTAACGTCATCAGCTCTAATAACGt < 1:390440/34‑1 (MQ=37) ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg > 1:225033/1‑36 (MQ=38) ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg < 1:749685/36‑1 (MQ=38) gCTGTTAACGTCATCAGCTCTAATAACGCGAAGTCa > 2:887531/1‑36 (MQ=37) gTTAACGTCATCAGCTCTAATAACGTGAAGTCAATa < 2:974576/36‑1 (MQ=38) aCGTCATCAGCTCTAATAACGTGAAGTCAATAGCCt < 1:386732/36‑1 (MQ=255) atcaGCTCTAATAACGTGAAGTCAATAGCCTGGCGt > 1:757064/1‑36 (MQ=38) | CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT > NC_012967/1248348‑1248410 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |