Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_012967	1,248,380	A→C	100%	D295E (GAT→GAG)	dhaM ←	dihydroxyacetone kinase subunit DhaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	1,248,380	0	A	C	89.4%	16.7 / ‑5.0	9	D295E (GAT→GAG)	dhaM	dihydroxyacetone kinase subunit DhaM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (3/5);  minor base T (1/0);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT > NC_012967/1248348‑1248410 | cTGGCTTCTGCTTTCGCTGTTAACGTCATCAGctct > 1:490537/1‑36 (MQ=38) cTGCTTTCGCTGTTAACGTCATCAGTTCtaataatg > 1:590347/1‑34 (MQ=37) cTGCTTTAGATGTTAACGTCATCAGCTCTAATAACg < 1:237367/36‑1 (MQ=19) ttcTTTCGCTGTTAACGTCATCAGCTCTAATAACGt < 1:390440/34‑1 (MQ=37) ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg > 1:225033/1‑36 (MQ=38) ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg < 1:749685/36‑1 (MQ=38) gCTGTTAACGTCATCAGCTCTAATAACGCGAAGTCa > 2:887531/1‑36 (MQ=37) gTTAACGTCATCAGCTCTAATAACGTGAAGTCAATa < 2:974576/36‑1 (MQ=38) aCGTCATCAGCTCTAATAACGTGAAGTCAATAGCCt < 1:386732/36‑1 (MQ=255) atcaGCTCTAATAACGTGAAGTCAATAGCCTGGCGt > 1:757064/1‑36 (MQ=38) | CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT > NC_012967/1248348‑1248410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑