Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_012967	3,289,962	Δ16 bp	100%	coding (3‑18/4461 nt)	gltB →	glutamate synthase large subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_012967	3289962	3289977	16	10 [0]	[0] 10	gltB	glutamate synthase large subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_012967	= 3289961	0 (0.000)	9 (0.940)	9/50	NT	100%	coding (2/4461 nt)	gltB	glutamate synthase large subunit
	?	NC_012967	3289978 =	0 (0.000)					coding (19/4461 nt)	gltB	glutamate synthase large subunit

GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_012967/3289927‑3289961 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTGAGAGGGATAACTGTGGTTTC  >  NC_012967/3289978‑3290001                                                             GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATC                         <  2:396819/36‑1      CCCGCAGAGCCTGGGGGAGGTTCACGATATCTTGAG                    <  1:321637/36‑1       CCGCAGAGCCTGGGGGAGGTTCACGATATCTTGAGA                   >  1:728798/1‑36        CGCAGAGCCTGGGGGAGGTTCACGATATCTTGAGAG                  >  2:733878/1‑36               CCTGGGGGAGGTTCACGATATCTTGAGAGGGATAAC           <  2:770294/36‑1                CTGGGGGAGGTTCACGATATCTTGAGAGGGTTAACT          >  2:188883/1‑36                  GGGGGAGGTTCACGATATCTTGAGAGGGATAACTGT        >  1:925597/1‑36                     GGAGGTTCACGATATCTTGAGAGGGATAACTGTGGT     <  1:500979/36‑1                       AGGTTCACGATATCTTGAGAGGGATAACTGTGGTTT   >  1:280225/1‑36                        GGTTCACGATATCTTGAGAGGGATAACTGTGGTTTC  <  2:968407/36‑1                                                             GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_012967/3289927‑3289961 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTGAGAGGGATAACTGTGGTTTC  >  NC_012967/3289978‑3290001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.