Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_012967	3,289,962	Δ16 bp	100%	coding (3‑18/4461 nt)	gltB →	glutamate synthase large subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_012967	3289962	3289977	16	10 [0]	[0] 10	gltB	glutamate synthase large subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_012967	= 3289961	0 (0.000)	9 (0.940)	9/50	NT	100%	coding (2/4461 nt)	gltB	glutamate synthase large subunit
	?	NC_012967	3289978 =	0 (0.000)					coding (19/4461 nt)	gltB	glutamate synthase large subunit

GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAG > NC_012967/3289927‑3289985 | gggTTCCCGCAGAGCCTGGGGGAGGTTCACGATATc < 2:396819‑M1/36‑2 (MQ=255) cccGCAGAGCCTGGGGGAGGTTCACGATATcttgag < 1:321637‑M1/36‑7 (MQ=255) ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga > 1:728798‑M1/1‑29 (MQ=255) cGCAGAGCCTGGGGGAGGTTCACGATATcttgagag > 2:733878‑M1/1‑28 (MQ=255) ccTGGGGGAGGTTCACGATATcttgagagggataac < 2:770294‑M1/36‑16 (MQ=255) cTGGGGGAGGTTCACGATATcttgagagggttaact > 2:188883‑M1/1‑20 (MQ=255) gggggAGGTTCACGATATcttgagagggataactgt > 1:925597‑M1/1‑18 (MQ=255) ggAGGTTCACGATATcttgagagggataactgtggt < 1:500979‑M1/36‑22 (MQ=255) aGGTTCACGATATcttgagagggataactgtggttt > 1:280225‑M1/1‑13 (MQ=255) ggTTCACGATATcttgagagggataactgtggtttc < 2:968407‑M1/36‑25 (MQ=255) | GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAG > NC_012967/3289927‑3289985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.