BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_012967 3,289,962 Δ16 bp 100% coding (3‑18/4461 nt) gltB → glutamate synthase large subunit

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_012967 3289962 3289977 16 10 [0] [0] 10 gltB glutamate synthase large subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012967 = 32899610 (0.000)9 (0.940) 9/50 NT 100% coding (2/4461 nt) gltB glutamate synthase large subunit
?NC_012967 3289978 = 0 (0.000)coding (19/4461 nt) gltB glutamate synthase large subunit

GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAG  >  NC_012967/3289927‑3289985
                                  |                        
gggTTCCCGCAGAGCCTGGGGGAGGTTCACGATATc                         <  2:396819‑M1/36‑2 (MQ=255)
     cccGCAGAGCCTGGGGGAGGTTCACGATATcttgag                    <  1:321637‑M1/36‑7 (MQ=255)
      ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga                   >  1:728798‑M1/1‑29 (MQ=255)
       cGCAGAGCCTGGGGGAGGTTCACGATATcttgagag                  >  2:733878‑M1/1‑28 (MQ=255)
              ccTGGGGGAGGTTCACGATATcttgagagggataac           <  2:770294‑M1/36‑16 (MQ=255)
               cTGGGGGAGGTTCACGATATcttgagagggttaact          >  2:188883‑M1/1‑20 (MQ=255)
                 gggggAGGTTCACGATATcttgagagggataactgt        >  1:925597‑M1/1‑18 (MQ=255)
                    ggAGGTTCACGATATcttgagagggataactgtggt     <  1:500979‑M1/36‑22 (MQ=255)
                      aGGTTCACGATATcttgagagggataactgtggttt   >  1:280225‑M1/1‑13 (MQ=255)
                       ggTTCACGATATcttgagagggataactgtggtttc  <  2:968407‑M1/36‑25 (MQ=255)
                                  |                        
GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAG  >  NC_012967/3289927‑3289985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.